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We narrowed to 14 results for: Gal4 UAS

Showing: 1 - 14 of 14 results

  1. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...cell-type specificity! The Gal4/UAS system At a more detailed level, the Gal4/UAS system is a transcription...Applications of the Gal4/UAS system There are a number of fancy ways to use the Gal4/UAS system, and you ...my recent paper to see the Gal4/UAS system in action (5). I use the Gal4/UAS system to knock down gene ...encoded downstream of the UAS sequence are only expressed when Gal4 is expressed. Gal4 expression can be regulated...Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple ...down. To do this, Drosophila geneticists use the Gal4/UAS system. This incredibly useful, yet simple system...to turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can therefore place GAL4 and GAL80...common binary system is the GAL4/UAS system isolated from yeast. In this system, UAS basal promoter expression...expression is low but is activated by GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- ...to intermediate expression from QUAS, as seen with GAL4/GAL80 and UAS. QF-mediated repression is reversible...phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila or development studies, you may ... Lee. LexA/lexAop is a complementary system to GAL4/UAS that functions in essentially the same manner,...activators of lexAop. This system is commonly used with GAL4/UAS to examine the expression of reporter genes, or...

  3. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...1):  GAL4-UAS, a transcription factor binding site. GAL4 expressed in the fly binds to the UAS binding...SPARC Figure 1: SPARC and SPARC2 use the GAL4-UAS system along with precisely truncated attP sites...

  4. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...Drosophila, where expression of Cre from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-...flies homozygous for a UAS-driven recombinase with flies heterozygous for tubulin-GAL4. For Dre, Flp, KD, ...50% of offspring carried both the recombinase and GAL4, indicating that the recombinases were inherited...In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity of this enzyme...

  5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...experimental model organism. I then described the Gal4/UAS system used by geneticists to study gene function...favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the available reagents do not match...by filtering by fly as species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...transactivator is used. UAS General expression mRNA Yeast promoter containing Gal4 binding sites, commonly... in Drosophila Specific Requires the presence of Gal4 gene to activate promoter. Ac5 General expression... be used independently or together. Regulated by GAL4 and GAL 80. TEF1 General expression mRNA Yeast...

  8. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...fly is the array of genetic tools, such as the GAL4/UAS and LexA system, that allows scientists to easily...but can be quite difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon...

  9. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...integration into the fly genome and are under the Gal4 inducible UAS promoter. Other vectors can be expressed ...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...able to bind to the upstream activation sequence (UAS) of its target gene, in this case a reporter gene...Although the original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and the lacZ reporter...the binding sites for the protein partner or the UAS/reporter gene, the same bait and prey libraries can...

  12. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... Wang, Han, et al. "cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans." Nature methods...2018, the lab expanded on this tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein...
Showing: 1 - 14 of 14 results