...-iGluSnFR), green (SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators.... Building on the previous generation of the SF-Venus-iGluSnFR (itself an improvement over iGluSnFR), ...1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857...

...it by your keys a day in advance. Dress for the venue and professionalism of the conference, and remember...poster session, or it may be held at an unfamiliar venue. Finding your poster board well ahead of time ensures...you’ll have extra time to practice at the actual venue. Introduce yourself: I always find it odd when I...

...of ECFP pairs with N173 derived from Cerulean or Venus Identification of new fluorescent protein fragments...Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow Venus pBiFC-VN173, pBiFC-VC155 Identification of new fluorescent...Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal fragment VN155(I152L), for use.... 2010 Chang-Deng Hu mVenus pET-BiFC contains both fragments of split mVenus (aa 155), includes I152L...Mulvihill DP. FEBS Lett. 2017 Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment...

...interest fused to the N-terminus of monomeric Venus mVenus C1 Yellow Mammalian Express a gene of interest fused... fused to the C-terminus of monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized...express a gene of interest fused between ECFP and Venus Other issues that can affect FRET measurements...including: mCerulean/mVenus, mCerulean/Amber, mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP ... of interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest ...

... called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca++ binding...reporter by using NanoLuc® Luciferase to excite the Venus fluorescent protein in conjunction with a membrane-inserted...

...of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology is based on the principle...

...from the correct layer.  Third, think about the venue you are using to present the image and information...

...other settings, including “conventional” academic venues such as scientific, educational or industry conferences...

...web searches; likewise, most in-person events and venues will have a solid understanding of their audience...

...found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be split into two halves, which are non-fluorescent...

...superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an A206K mutation... these constructs and they are designated mGFP, mVenus, etc. A word of caution is needed because FPs not...

...out information about the particular audience and venue before tailoring each presentation appropriately...

...the acceptor fluorophore, a circularly permuted Venus. In the case of pHlash, the signal from the fluorophore...host-tropism. These tools open up many powerful avenues through which one can examine molecular events ...

...as a nonprofit under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit... are able to fulfill our mission by generating revenue through distribution of research materials. Our..." is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution... nonprofits should have some level of positive revenue to build reserves so the company is sustainable...The difference is what nonprofits do with their revenue. A nonprofit organization cannot distribute profits...

... For example, the APT1-mVenus fusion localizes at the Golgi, whereas the mVenus-APT1 fusion does not (... (figure 2). In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization, is occluded...therefore this protein is mislocalized. The APT1-mVenus fusion is clearly a better choice to work with....

... fused to mVenus linked to a mitochondrial localization sequence TOM20 (pTriEX Tom20 mVenus BoPD). In ...

...expressed a fusion of keratin with mGold (top) and mVenus (bottom). Cells were imaged every 30 seconds for... up to 5-fold more photostable and as bright to mVenus, the parent YFP, and is the most photostable YFP...

...RNA without tagging,” she said. “It opens a new avenue of modifying and manipulating endogenous eukaryotic...

...silencer. The current work also proposes promising avenues for future engineering to shift the spectra and...

...not make changes to the genome, thus providing an avenue for temporary/reversible CRISPR modification. In...