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Addgene
Showing: 1 - 20 of 48 results
  1. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...construct consisting of Venus-5aa linker-Cerulean-5aa linker-Venus-6aa linker-Venus V5V Anisotropy/brightness...Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus via a 17 amino acid...linker-Cerulean-6aa linker-Venus VCA Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa...Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa linker-Venus ACAV Heterotetrameric construct...linker-Venus ACVA Heterotetrameric construct consisting of Amber-5aa linker-Cerulean-5aa linker-Venus-6aa...interest fused to the N-terminus of monomeric Venus mVenus C1 Yellow Mammalian Express a gene of interest fused...pmTurquoise2-T2A-Venus(L68V) Brightness standard used a negative control (no FRET) with pmVenus(L68V)-mTurquoise2...
  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...-iGluSnFR), green (SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators.... Building on the previous generation of the SF-Venus-iGluSnFR (itself an improvement over iGluSnFR), ...1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857...
  3. Adenovirus Plasmids

    Type
    Collection
    ...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid...plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular inserts that ...
  4. Optogenetics AAV Preps

    Type
    Collection
    ...pAAV-CAG-DIO-PPO-Venus CAG PPO Venus Cre dependent 5 Bruchas 139505 pAAV-Ef1a-DIO-PPO-Venus EF1a PPO Venus Cre dependent...Excitatory: Wild-type ChR2 20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2...
  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...it by your keys a day in advance. Dress for the venue and professionalism of the conference, and remember...poster session, or it may be held at an unfamiliar venue. Finding your poster board well ahead of time ensures...you’ll have extra time to practice at the actual venue. Introduce yourself: I always find it odd when I...
  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...of ECFP pairs with N173 derived from Cerulean or Venus Identification of new fluorescent protein fragments...Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow Venus pBiFC-VN173, pBiFC-VC155 Identification of new fluorescent...Deng X, Hu CD. Biotechniques. 2006 Chang-Deng Hu Venus Improved N-terminal fragment VN155(I152L), for use.... 2010 Chang-Deng Hu mVenus pET-BiFC contains both fragments of split mVenus (aa 155), includes I152L...Mulvihill DP. FEBS Lett. 2017 Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment...
  7. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...interest fused to the N-terminus of monomeric Venus mVenus C1 Yellow Mammalian Express a gene of interest fused... fused to the C-terminus of monomeric Venus pCEP4YPet-MAMM Yellow Mammalian Expresses mammalian optimized...express a gene of interest fused between ECFP and Venus Other issues that can affect FRET measurements...including: mCerulean/mVenus, mCerulean/Amber, mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP ... of interest fused to the C-terminus of Amber mVenus N1 Yellow Mammalian Express a gene of interest ...
  8. Luciferase Plasmids

    Type
    Collection
    ...Wanker 124701 pLenti-PGK-Venus-Akaluc (neo) Akaluc hPGK Lentiviral expression of Venus-Akaluc Roland Friedel...Kay 140328 pLenti-PGK-Venus-Fluc (puro) Firefly hPGK Lentiviral expression of Venus-firefly luciferase Roland...intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. ...an Ala-Glu-Leu linker to the circularly permuted Venus fluorophore. Learn more about pHlash sensor in our...
  9. Cre-lox system

    Type
    Collection
    ...coexpression Retroviral Littman 24333 ins-cre_cryaa-Venus Cre-Venus fusion insulin Zebrafish Stainier 24593 AAV-pgk-Cre...mCherry-Cre fusion and Venus Elastase Zebrafish Stainier 84033 pHD157 mCherry-Cre fusion and Venus fabp10 Zebrafish...inducible. PBAD promoter Bacterial Richmond 112614 pVHC Venus and Cre-ERT2 with MCS for inserting promoter none... promoter none Mammalian Heller 112618 pCAG-VHC Venus, Cre-ERT2 CAG Mammalian Heller 112619 pCAG-EHC Emerald...CAG Mammalian Heller 112622 pVHC_PGKneoLox2DTA.2 Venus, Cre-ERT2, targeting vector with MCS for homology...
  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...VN-Tau (wt) MAPT Venus CMV Parkinson's, FTD Tiago Outeiro 87369 Tau (wt) -VC MAPT Venus CMV Parkinson's...P301L)-VC MAPT Venus CMV Parkinson's, FTD Tiago Outeiro 87634 VN-Tau (P301L) MAPT Venus CMV Parkinson'...Synuclein SNCA Venus CMV Parkinson's Bradley Hyman 89471 alpha Synuclein-VC SNCA Venus CMV Parkinson's...Synuclein SNCB Venus CMV Lewy body dementia Tiago Outeiro 89484 beta Synuclein-VC SNCB Venus CMV Lewy body...89755 VN-Sph1 SNCAIP Venus CMV Parkinson's Tiago Outeiro 89757 VN-SOD1 SOD1 Venus CMV ALS Tiago Outeiro...-SorLA-v1 SORL1 Venus CMV Alzheimer's Johanna Ivaska 154900 pDEST-SorLA-v2 SORL1 Venus CMV Alzheimer's...175060 pQ54-VC HTT Venus, mCherry CMV Huntington's Arnold Boersma 175061 pQ39-VC HTT Venus, mCherry CMV Huntington's...
  11. Biosensor AAV Preps

    Type
    Collection
    ...Looger 106183 pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S Syn SF-Venus-iGluSnFR.A184S none Cre dependent ... Looger 106185 pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A Syn SF-Venus-iGluSnFR.S72A none Cre dependent 1...
  12. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... called CalFlux VNT. CalFlux VNT is composed of Venus fluorescent protein, the Troponin C Ca++ binding...reporter by using NanoLuc® Luciferase to excite the Venus fluorescent protein in conjunction with a membrane-inserted...
  13. Clear and Accessible Writing

    Type
    Blog Post
    ...from the correct layer.  Third, think about the venue you are using to present the image and information...
  14. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double...pAAV-hsyn-Jaws-KGC-GFP-ER2 Ed Boyden AV-9-20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double...Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-5-20949P 20949-AAV5 pAAV-double...
Showing: 1 - 20 of 48 results