We narrowed to 37 results for: gateway

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Jan. 12, 2017, 3:30 p.m.

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Dec. 8, 2016, 3:30 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

In the second episode of our Hot plasmids podcast, learn about new RFPs, AAV tools, and plasmids for generating transgenic zebrafish.

Sept. 8, 2017, 7:52 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

Where did plasmids come from? Learn more about natural plasmids, the benefits they offer cells, and how scientists have adapted them into laboratory tools.

Oct. 8, 2024, 1:15 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Learn more about the common E. coli strains used in the lab.

Nov. 7, 2014, 2:56 p.m.

Designing a cloning experiment? Read this blog post to learn about different positive and negative selection strategies you can take to find the clones you're looking for.

Aug. 22, 2019, 12:43 p.m.

SPARC is a tool that labels a fraction of a genetically-defined subset of cells. The result: easy visualization of individual, non-overlapping cells.

May 21, 2020, 1:15 p.m.

New to plasmids? Learn the basics of plasmids in this blog post and find out about the parts of a plasmid, how they are made, and how scientists can use them in the lab.

April 2, 2020, 6:17 p.m.

Learn about the importance of antibiotic resistance genes in plasmids. How they work, how to use them, and some great tips.

Jan. 30, 2014, 3:29 p.m.

A review of a new brain to gut neural fiber technology from the Anikeeva and Bohorquez labs.

Aug. 31, 2023, 1 p.m.