Exploring Applications of the Bioluminescent HiBiT Tag
Type
Blog Post
Published
Oct. 2, 2025, 1:15 p.m.
...which, upon binding to its complementary partner LgBiT, reconstitutes a fully functional luciferase enzyme...HiBiT-TP53 expression was detected by blotting with LgBiT, and the authors used a p53 response-element NanoLuc...from cells expressing ADAR1-HiBiT were added to LgBiT-expressing hepatocytes or co-cultured across very...Due to the lack of membrane permeability of the LgBiT protein, it only binds HiBiT still present on the...heteromer (Peach et al., 2021). They used HiBiT and LgBiT fusion proteins to track how specific modifications...