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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4 results for: lgbit

Showing: 1 - 4 of 4 results

  1. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...which, upon binding to its complementary partner LgBiT, reconstitutes a fully functional luciferase enzyme...HiBiT-TP53 expression was detected by blotting with LgBiT, and the authors used a p53 response-element NanoLuc...from cells expressing ADAR1-HiBiT were added to LgBiT-expressing hepatocytes or co-cultured across very...Due to the lack of membrane permeability of the LgBiT protein, it only binds HiBiT still present on the...heteromer (Peach et al., 2021). They used HiBiT and LgBiT fusion proteins to track how specific modifications...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...unfolds under tension. When this happens, BiT and the LgBiT luciferase fragment (expressed separately) assemble...Left: Mechanism of the PILATeS tension sensor. The LgBiT (cyan) fragment of luciferase binds to the BiT Tag... Titin I10 unfolds under tension. Upon binding, LgBiT and BiT Tag catalyze a luminescent reaction with...

  3. Promega Plasmid Collection

    Type
    Collection
    ...containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target...using tags such as NanoLuc, HaloTag, SmBiT, and LgBiT. Ordering & Availability Plasmids from the Promega...The technology includes NanoLuc, HaloTag, SmBiT, LgBiT, HiBiT, and more. Luciferase Reporters Luciferase...complementation reporter system composed of a Large BiT (LgBiT; 18 kDa) subunit and a small complementary peptide...where each protein of interest is fused to either LgBiT or SmBiT and expressed in live cells. When the two...precise protein detection. It can be paired with LgBiT for bioluminescent assays or with the high-affinity...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...window) : The NanoLucĀ® enzyme is split into Large (LgBiT; 18 kDa) and Small (SmBiT; 11 amino acids) fragments... assays. Each target protein is fused to either LgBiT or SmBiT and upon interaction, the NanoBiT subunits...enzyme, generating luminescence. Find plasmids with LgBit and SmBit . Cluc-FKBP and FRB-Nluc : Split firefly...
Showing: 1 - 4 of 4 results