We narrowed to 25 results for: loxP

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Jan. 13, 2015, 3:47 p.m.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

April 28, 2016, 2:30 p.m.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Dec. 1, 2016, 3:30 p.m.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Oct. 19, 2017, 1:54 p.m.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Nov. 2, 2021, 1:15 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

April 5, 2018, 12:42 p.m.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Feb. 16, 2023, 7:05 p.m.

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Aug. 6, 2019, 12:55 p.m.

Learn about the common techniques to creating engineered mouse models at embryonic stages and the pros and cons of different genome editing techniques.

July 11, 2019, 1:26 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

May 31, 2016, 3:30 p.m.

Learn how FLEx and optogenetics combined can be combined to control gene expression with high spatial and temporal resolution.

Oct. 17, 2019, 12:28 p.m.

When using AAV for precise neuronal targeting consider AAV tropism and administration route, cell-type specific expression, and combining different approaches to increase targeting specificity further.

Nov. 27, 2019, 2:03 p.m.

Stochastic multicolor labeling is a powerful tool for discriminating between neurons. Learn about how a new system named Tetbow (Tetracycline transactivator Brainbow) can bring brighter multicolor labeling to neuronal tracing experiments.

Jan. 24, 2019, 2:24 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

April 17, 2018, 1:37 p.m.

Connie Cepko's lab at Harvard University has developed transcription factor and CRE recombinase variants activated by GFP. Learn more inside!

Nov. 24, 2015, 3:30 p.m.

Learn how the Gradinaru Lab at Caltech developed AAV vectors to target the peripheral and central nervous systems.

Sept. 28, 2017, 2:01 p.m.