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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5 results for: plko.1

Showing: 1 - 5 of 5 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse... experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David...target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its name ...manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the...

  3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited by David Root of ...How would the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem...

  4. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...information and a detailed protocol on how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre...here. Cloning and expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by ...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our complete guide...
Showing: 1 - 5 of 5 results