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We narrowed to 5 results for: plko.1

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Showing: 1 - 5 of 5 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...expression vector Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning...PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2... lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning...AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-1 plasmid...vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression...N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...

  2. Lentivirus Plasmids

    Type
    Collection
    ...expression . ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puromycin...24150 for hygromycin selection. Bob Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty...Includes a stuffer for easy cloning. David Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker...selection. Christophe Benoist and Diane Mathis 21915 Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin...coexpression. Didier Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression... 21374 for dTomato version. Bryan Welm 21916 Tet-pLKO-neo 3rd Inducible expression of shRNA with neomycin...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols... design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference. Created...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool to control...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...though the DNA-binding profile is reversed (Figure 1). However, rtTA can induce much stronger expression...Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression...selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning... selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible...Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector with blasticidin selection...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SOD1 EF1-alpha ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His...HA-KIF5A KIF5A HA CMV ALS Rosalind Segal 167294 pLKO.1 puro_shTBK1_3185 TBK1 ALS Agnel Sfeir 167354 pOPINB-hRNF216... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [...3XHA CHMP2B HA CMV ALS Franz-Ulrich Hartl 242422 pLKO.1 mouse Chmp2b CHMP2B hU6 ALS Franz-Ulrich Hartl ...Cookson 13321 pGEX5X.1 PINK1 WT PINK1 GST tac Parkinson's Mark Cookson 13322 pGEX5X.1 PINK1 KD PINK1 GST... His, V5 EF-1 alpha Parkinson's Mark Cookson 25081 pDEST51-LRRK2-R1441C LRRK2 His, V5 EF-1 alpha Parkinson's...
Showing: 1 - 5 of 5 results