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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 6 results for: plko.1

Showing: 1 - 6 of 6 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Mammalian Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning...vector for mammalian genome editing Tet-pLKO-puro and Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression...PGK driven puromycin Hygromycin Mammalian, Varies pLKO.1 hygro - Lentiviral shRNA expression lentiCRISPRv2... lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning...AdEasier®-1 cells (strain) - Bacterial strain that contains AdEasy®-1 plasmid... Backbones Neomycin (G418) Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression ... for gene expression Thy1.1 Mammalian pLKO.3 Thy1.1 - Lentiviral shRNA expression Gentamicin...

  2. Lentivirus Plasmids

    Type
    Collection
    ...lenti". ID Plasmid Generation Description PI 8453 pLKO.1 puro 3rd U6-driven shRNA empty plasmid with puro...plasmid 24150 for hygro resistance. Weinberg 10878 pLKO.1 – TRC cloning plasmid 3rd U6-driven shRNA empty...; includes a stuffer for easy cloning Root 14748 pLKO.3G 3rd U6-driven shRNA empty plasmid with EGFP marker...for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin...plasmid 21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin ...working with lentivirus: these viruses are based on HIV-1, which may require additional lab biosafety procedures... EGFP coexpression. Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression...selection. TetR H1-2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning... selection. TetR H1-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible...and releases tet O, enabling transcription (Figure 1). While TetR and tet O could be a basic tool to control...skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR...although the DNA-binding profile is reversed (Figure 1). However, rtTA can induce stronger expression of ...Transactivator PI 26429 pLenti CMV rtTA3 Blast (w756-1) Lentiviral Tet-On vector with blasticidin selection...

  4. Mammalian RNAi Tools

    Type
    Collection
    ...Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols... design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference. Created...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Lentiviral BsmBI yes, cut S. pyogenes EGFP Ebert pLKO.1-puro U6 sgRNA BfuAI stuffer 50920 Mammalian/Lentiviral... see plasmid page yes, cut S. pyogenes BFP Kuhn pLKO.1-puro U6 sgRNA BfuAI large stuffer 52628 Mammalian...Worm HindIII none S. pyogenes de Bono sgRNA with rpr-1 promoter 48961 Worm EcoRI none S. pyogenes de Bono...Zebrafish BseRI none S. pyogenes Zon pCRISPomyces-1 61736 Bacteria BbsI yes, cut S. pyogenes Apramycin...Puro Zoldos pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110) 71707 Mammalian hU6 yes, cut S. pyogenes Draetta...expression of Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent promoters. sgRNAs...for the expression of two gRNAs from Drosophila U6:1 and U6:3 promoters. Bullock pCFD5 Drosophila Utilizes...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SOD1 EF1-alpha ALS George Church 222870 pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [... Leong Ho 102970 pLKO.1P shFXN_1 FXN Friedreich ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich...HA-KIF5A KIF5A HA CMV ALS Rosalind Segal 167294 pLKO.1 puro_shTBK1_3185 TBK1 ALS Agnel Sfeir 167354 pOPINB-hRNF216...Cookson 13321 pGEX5X.1 PINK1 WT PINK1 GST tac Parkinson's Mark Cookson 13322 pGEX5X.1 PINK1 KD PINK1 GST... His, V5 EF-1 alpha Parkinson's Mark Cookson 25081 pDEST51-LRRK2-R1441C LRRK2 His, V5 EF-1 alpha Parkinson's...
Showing: 1 - 6 of 6 results