We narrowed to 5 results for: broad host range
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TypeCollection...Additionally, these viruses are able to target a broad range of dividing and non-dividing cell types with ...retroviruses , adenoviruses do not integrate into the host genome. Disadvantages of adenoviruses include transient...
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CRISPR References and Information
TypeCollection...human, mouse, and rat reference genomes. Hosted by the Broad GPP and developed by the John Doench , David...sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed...gene inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants,...and cytosine (CBE) base editors. Includes a wide range of reference genomes, including animals, plants,...for off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab... -
Luciferase Plasmid Collection
TypeCollection...Operon Bacterial expression of luciferase from broad host range plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo...dynamic range, and resistance to photobleaching has made luciferase a common choice in assays ranging from...bioluminescence found in a diverse number of organisms, ranging from bacteria to aquatic animals to insects. Luciferase...of expression. Scientists have developed a wide range of luciferase-based plasmid systems for many different...brighter signal and emission in the near-infrared range, enabling enhanced bioluminescence imaging in vivo...toolkit to assess the proteostasis status in a wide range of experimental systems. LumiLuc and LumiScarlet... -
Zhang Lab CRISPR Page
TypeCollection...invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas)...vectors that can be combined with Cas9 in a wide range of applications. Full references are below. The ... studying the function of genetic elements. To broadly enable the application of Cas9 in vivo and ex vivo... -
CRISPR Guide
TypeCollection... DNA, which scientists have modified for a wide range of genome engineering techniques . Collectively,... often found in plants, expanding the targeting range for plant genome engineering. Read more about Cas9...non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes TTTV (V = A.... R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum inhibitor of CRISPR-Cas9. Cell , 170 (6...& Liu, D. R. (2018). Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature...screens in E. coli identify essential genes and phage host factors. PLOS Genetics . 14 (11), e1007749. PMID...with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nature Biotechnology...