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Addgene
Showing: 1 - 20 of 20 results

  1. Cre-lox system

    Type
    Collection
    ...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...Placing a stop codon with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional... gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression promotes shRNA... and pSicoR-mCh-empty Lentiviral Jacks 11584 Lox-Stop-Lox TOPO Cre activates gene of interest by removing...generated at the loxP sites. For a more thorough introduction, check out Addgene’s Cre-lox blog post . Based...shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre...

  2. Mammalian RNAi Tools

    Type
    Collection
    ...as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To ...Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578...Nat. Methods. 2006. Sep;3(9):682-8. PubMed . Cre-lox-regulated conditional RNA interference from transgenes...

  3. Lentivirus Plasmids

    Type
    Collection
    ...selection. Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns...turns on shRNA expression. See article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP...

  4. Plasmid Collections

    Type
    Collection
    ...Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins...

  5. Validated gRNA Sequences

    Type
    Collection
    ...Winslow Lox-STOP-Lox synthetic GCGTATAGCATACATTATACG 66586 cut S. pyogenes 26018130 Xue Lox-STOP-Lox synthetic...GGTTTTGGACACTGGAACCG 49331 cut S. pyogenes 24326186 Liu near LoxP sites synthetic CGAAGTTATATTAAGGGTTC 69992 cut S...

  6. Rett Syndrome

    Type
    Collection
    ... PMID: 35148843 Joost Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking exon 1,2,3 C57BL/6 Mouse...

  7. Deisseroth INTRSECT Collection

    Type
    Collection
    ...and adding recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The...

  8. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen AV-1-PV3509...Svoboda AV-1-20297P 20297-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Karl Deisseroth...Deisseroth AV-1-20298P 20298-AAV1 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Karl Deisseroth AV-1-20938M...Deisseroth AV-5-20297P 20297-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Karl Deisseroth...Deisseroth AV-5-20298P 20298-AAV5 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Karl Deisseroth AV-5-20938M...Svoboda AV-9-20297P 20297-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA Karl Deisseroth...Deisseroth AV-9-20298P 20298-AAV9 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA Karl Deisseroth AV-9-20938M...

  9. Optogenetics AAV Preps

    Type
    Collection
    ...Deisseroth Excitatory: ChR2/H134R 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R... 5, 8, 9, rg* Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP...Inhibitory: eNpHR and variants 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent...

  10. Retrograde AAV viral preps

    Type
    Collection
    ...Control Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses...Norepinephrine sensor Li Optogenetics 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Activator,... Optogenetics Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...

  11. Brain Initiative Collection

    Type
    Collection
    ... Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal...179459-AAV1 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE Double floxed soma and AIS-localized genetically encoded voltage...St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE Double floxed genetically encoded voltage indicator (GEVI) JEDI...

  12. Retrovirus Plasmids

    Type
    Collection
    ...puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE MSCV Conditional overexpression...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and fluorescence assisted sorting ...2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting ...

  14. Genetic Code Expansion

    Type
    Collection
    ...Mut1-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...Mut2-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...

  15. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Flp recombinases to remove genes flanked by either loxP or frt sites respectively, and empty vectors to ...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ...Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that enables precise temporal and spatial...

  17. Control AAV Preps

    Type
    Collection
    ...PHP.eB, PHP.S Boyden 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry or nls-EGFP Cre dependent...

  18. Caltech Systemic Capsids

    Type
    Collection
    ...Activator ChR2 Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...
Showing: 1 - 20 of 20 results