...proceeds. One popular lox-stop-lox plasmid is from Tyler Jack's lab: Lox-Stop-Lox TOPO.  Cre-dependent ...tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox system is a technology that...translocation event is generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established...placing a stop codon with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream...Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo...Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to Cre-lox, and has...embryonic lethality. To facilitate the use of Cre-lox technology, transgenic mice have been constructed...

...have two pairs of lox sites which are part of a dual lox system called FLEX or DIO. Lox sites are recognized...transfer plasmid, but are often seen in them.  Lox sites Lox sites, in combination with the Cre recombinase...spatially and temporally control transgene expression. Lox sites are directional 34 bp sequences that can flank...first flips the sequence between the first pair of lox sites, and then excise the sequence between the second...transgene. By restricting the availability of Cre, lox sites lets researchers control the spatial and temporal...activation of a transgene. Figure 2: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis Virus (WHP...

...continued utility of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick...quick primer on how Cre-lox recombination works. Advances in Cre-lox recombination have included inducible...manipulation systems remain valuable to biology. Cre-lox recombination, discovered in the 1980s, is one of...expression, especially in in vivo models, and new Cre-lox based technologies are still being developed today...tamoxifen-responsive CreERT, the coupling of Cre-lox with FLP-FRT recombination to generate additional...Cellular Biology, harnessed these attributes of Cre-lox to create the Brainbow mouse neural labeling system... cre-lox...

...Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox Plasmids... in a similar fashion as between loxP sites (For details see Cre-lox blog post). Optimizing FLP FRT through... use the Cre-lox or one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex... contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful site specific...developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional... cre-lox...recombination between those sites. FRT sites and loxP sites differ at the nucleotide level but share an...

...heterozygous for each mutated allele.  Cre-lox expressing strains Cre-lox strains are a common example of mice...Cre-lox Read our genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read...expressing both Cre and LoxP occurs. But how are these mice made to begin with? Cre-lox mice are made from ...cross mice to create double knockout lines and Cre-lox lines, and how to properly control for genetically...recombination at the floxed site, your control should be littermates that express Cre but not loxP. With this two-part...that expresses Cre with a strain that expresses a floxed gene of interest. Cre may be expressed globally... is a dominant phenotype, while you’ll want the floxed gene to be homozygous in order for Cre-mediated...

...recombinase, which induces recombination at LoxP sites. Placing a lox-STOP-lox cassette upstream of a gene of interest...T-DDOGs can be used in combination with green-red Cre-lox systems. T-DDOGs can also be designed with other ...121. PubMed PMID: 22157958. Addgene Resources Cre-lox Resource Guide Fluorescent Protein Resource Guide... cre-lox...

... recombinase-dependent vector designs used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF) sequence prior to...unused (loxp) sites will be flanked by two lox sites now in the same orientation. Next, the loxp site is... to the system it binds one pair of lox sites, let's say the loxp pair, and flips the transgene into the...Cre-recombinase binds to either pair of lox sites, let's say the lox2272, that are in the opposite orientation...Recombinase-dependent vectors utilize pairs of specific sequences, lox (recognized by Cre) or frt (recognized by Flp), to... hypothesis, they randomly scrambled one pair of lox sites and either used the same sequence to flank ... in one loxp site and one lox2272 site on either end of the transgene. Since loxp and lox2272 can not ...

...Cre-lox Read our genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read..., conditional knockout/knockin mice, use the Cre-lox recombination system to express the transgene in ... expression of the target gene. In addition, Cre-lox systems can be used to invert a segment of DNA or...recombination of DNA sequences located between two loxP sites. The loxP sites are present at both ends of a gene... vector, creating the floxed gene segment. Crossing of mice containing a floxed gene to a Cre-expressing...recombination. To generate a conditional knockout mouse, loxP sites are inserted directly into the genome to flank...gene in the targeting vector. The stop cassette is floxed, so Cre-mediated recombination deletes the stop...

... after selection using the Cre/Lox recombination system. After “floxing” the resistance gene through the...conditional knockout, researchers often use the Cre/Lox system described earlier. In this case, you design....org Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about CRISPR  ...NeoR only and leave exon 2 floxed, as seen in line 4 of Figure 4. Since the loxP sites are located in intronic...template. These templates can include loxP sites to create conditional floxed alleles. Although CRISPR is very... the insertion of flanking LoxP sequences, you can remove the gene through the addition of Cre recombinase...this construct, the neomycin resistance gene is floxed so that it can be removed post-selection through...

...Learn more about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This...Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can be used to induce... to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)...

...Cre-lox recombination is an incredibly useful molecular biology tool, but like any biological system,...  cre-lox...Cre may induce recombination at pseudo- or cryptic loxP sites (estimated to occur at a frequency of 1.2 ... systems, Cre itself, without the presence of a floxed construct, may produce a phenotype. This problem...458-65. doi:10.1007/s004270100167. PubMed. Cryptic loxP sites in mammalian genomes: genome-wide distribution...

...application of iTOP Having tested iTOP using Cre-lox recombination, D’Astolfo et al. were eager to see... cre-lox...series of primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem...

...   Recombinases Recombinase systems such as Cre-lox allow for more accurate cell and tissue targeting...functional Cre enzyme that induces recombination of lox sites. This system takes advantage of the extensive...Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey 50457 AAV2 pAAV-hSyn-DIO-EGFP...

...Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli...Tetracycline (Tet) On Expression Systems at Addgene Cre-lox Guide and Plasmids     ...

...and reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems (ex: tamoxifen). As the... the name suggests, CreLite combines the Cre/lox system with optogenetics. Cre activity is controlled ...

...Cre-lox Plasmids Addgene's Blog: Cre-lox Addgene's Blog: FLEx Vectors Cre-containing Plasmids loxP Constructs...Placing a stop codon with loxP sites on either side (often called a “lox-stop-lox” or “LSL” cassette) upstream... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional... gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression promotes shRNA... and pSicoR-mCh-empty Lentiviral Jacks 11584 Lox-Stop-Lox TOPO Cre activates gene of interest by removing...generated at the loxP sites. For a more thorough introduction, check out Addgene’s Cre-lox blog post . Based...shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre...

...bacteriophage integrase phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been successfully...zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments." Methods in cell biology...

...using Cre-Lox mouse models in which the endogenous MECP2 gene was disrupted and silenced by a Lox-Stop cassette...

...as well as those that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To ...Lentiviral shRNA Lentiviral vectors for conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578...Nat. Methods. 2006. Sep;3(9):682-8. PubMed . Cre-lox-regulated conditional RNA interference from transgenes...

...system for controlling gene expression such as Cre-lox, may be sufficient to overcome the transport issues...to produce neuron specific transfection using Cre-lox, this new method could allow for labeling of neurons...