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Ekker Lab TALEN Kit
(Kit # 1000000038 )

Depositing Lab:   Stephen Ekker

This kit contains all 256 possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs.

This kit consists of three 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$800 USD + shipping

Available to academics and nonprofits only.

Original Publication

High Efficiency In Vivo Genome Engineering With a Simplified 15-RVD GoldyTALEN Design. Ma AC, Lee HB, Clark KJ and Ekker SC. PLoS One 2013 May 29;8(5):e65259. doi: 10.1371/journal.pone.0065259. PubMed .

Description

This Complete pFUS_B4 Collection contains all 256 possible combinations of pFUS_B4 clones built with TALE repeat modules contain RVDs NI, HD, NN and NG. The collection is aimed to work in complement with the Golden Gate TALEN and TAL Effector Kit 2.0 to assemble highly active 15-RVD GoldyTALENs without the need for building individual pFUS_B4 intermediates.

Besides the RCIscript-GoldyTALEN backbone which is highly active in zebrafish, the collection is also compatible with other Golden Gate TALEN backbones.

Ekker_lab_TALEN.jpg

Kit Documentation

Icon Ekker Lab TALEN Kit (1.3 MB)

Protocol

Download a Protocol for synthesizing 15-RVD TALENs:

Icon Ekker lab protocol (60.5 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The complete pFUS_B4 collection for Golden Gate assembly of 15-RVD TALENs was a gift from Stephen Ekker (Addgene kit # 1000000038)"

For your Reference section:
High Efficiency In Vivo Genome Engineering With a Simplified 15-RVD GoldyTALEN Design. Ma AC, Lee HB, Clark KJ and Ekker SC. PLoS One 2013 May 29;8(5):e65259. doi: 10.1371/journal.pone.0065259. PubMed .

Ekker Lab TALEN kit - #1000000038

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Spectinomycin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 pFUSB4_AAAA
Spectinomycin
Plate 1 / A / 2 pFUSB4_AAGA
Spectinomycin
Plate 1 / A / 3 pFUSB4_ACAA
Spectinomycin
Plate 1 / A / 4 pFUSB4_ACGA
Spectinomycin
Plate 1 / A / 5 pFUSB4_AGAA
Spectinomycin
Plate 1 / A / 6 pFUSB4_AGGA
Spectinomycin
Plate 1 / A / 7 pFUSB4_ATAA
Spectinomycin
Plate 1 / A / 8 pFUSB4_ATGA
Spectinomycin
Plate 1 / A / 9 pFUSB4_CAAA
Spectinomycin
Plate 1 / A / 10 pFUSB4_CAGA
Spectinomycin
Plate 1 / A / 11 pFUSB4_CCAA
Spectinomycin
Plate 1 / A / 12 pFUSB4_CCGA
Spectinomycin
Plate 1 / B / 1 pFUSB4_AAAC
Spectinomycin
Plate 1 / B / 2 pFUSB4_AAGC
Spectinomycin
Plate 1 / B / 3 pFUSB4_ACAC
Spectinomycin
Plate 1 / B / 4 pFUSB4_ACGC
Spectinomycin
Plate 1 / B / 5 pFUSB4_AGAC
Spectinomycin
Plate 1 / B / 6 pFUSB4_AGGC
Spectinomycin
Plate 1 / B / 7 pFUSB4_ATAC
Spectinomycin
Plate 1 / B / 8 pFUSB4_ATGC
Spectinomycin
Plate 1 / B / 9 pFUSB4_CAAC
Spectinomycin
Plate 1 / B / 10 pFUSB4_CAGC
Spectinomycin
Plate 1 / B / 11 pFUSB4_CCAC
Spectinomycin
Plate 1 / B / 12 pFUSB4_CCGC
Spectinomycin
Plate 1 / C / 1 pFUSB4_AAAG
Spectinomycin
Plate 1 / C / 2 pFUSB4_AAGG
Spectinomycin
Plate 1 / C / 3 pFUSB4_ACAG
Spectinomycin
Plate 1 / C / 4 pFUSB4_ACGG
Spectinomycin
Plate 1 / C / 5 pFUSB4_AGAG
Spectinomycin
Plate 1 / C / 6 pFUSB4_AGGG
Spectinomycin
Plate 1 / C / 7 pFUSB4_ATAG
Spectinomycin
Plate 1 / C / 8 pFUSB4_ATGG
Spectinomycin
Plate 1 / C / 9 pFUSB4_CAAG
Spectinomycin
Plate 1 / C / 10 pFUSB4_CAGG
Spectinomycin
Plate 1 / C / 11 pFUSB4_CCAG
Spectinomycin
Plate 1 / C / 12 pFUSB4_CCGG
Spectinomycin
Plate 1 / D / 1 pFUSB4_AAAT
Spectinomycin
Plate 1 / D / 2 pFUSB4_AAGT
Spectinomycin
Plate 1 / D / 3 pFUSB4_ACAT
Spectinomycin
Plate 1 / D / 4 pFUSB4_ACGT
Spectinomycin
Plate 1 / D / 5 pFUSB4_AGAT
Spectinomycin
Plate 1 / D / 6 pFUSB4_AGGT
Spectinomycin
Plate 1 / D / 7 pFUSB4_ATAT
Spectinomycin
Plate 1 / D / 8 pFUSB4_ATGT
Spectinomycin
Plate 1 / D / 9 pFUSB4_CAAT
Spectinomycin
Plate 1 / D / 10 pFUSB4_CAGT
Spectinomycin
Plate 1 / D / 11 pFUSB4_CCAT
Spectinomycin
Plate 1 / D / 12 pFUSB4_CCGT
Spectinomycin
Plate 1 / E / 1 pFUSB4_AACA
Spectinomycin
Plate 1 / E / 2 pFUSB4_AATA
Spectinomycin
Plate 1 / E / 3 pFUSB4_ACCA
Spectinomycin
Plate 1 / E / 4 pFUSB4_ACTA
Spectinomycin
Plate 1 / E / 5 pFUSB4_AGCA
Spectinomycin
Plate 1 / E / 6 pFUSB4_AGTA
Spectinomycin
Plate 1 / E / 7 pFUSB4_ATCA
Spectinomycin
Plate 1 / E / 8 pFUSB4_ATTA
Spectinomycin
Plate 1 / E / 9 pFUSB4_CACA
Spectinomycin
Plate 1 / E / 10 pFUSB4_CATA
Spectinomycin
Plate 1 / E / 11 pFUSB4_CCCA
Spectinomycin
Plate 1 / E / 12 pFUSB4_CCTA
Spectinomycin
Plate 1 / F / 1 pFUSB4_AACC
Spectinomycin
Plate 1 / F / 2 pFUSB4_AATC
Spectinomycin
Plate 1 / F / 3 pFUSB4_ACCC
Spectinomycin
Plate 1 / F / 4 pFUSB4_ACTC
Spectinomycin
Plate 1 / F / 5 pFUSB4_AGCC
Spectinomycin
Plate 1 / F / 6 pFUSB4_AGTC
Spectinomycin
Plate 1 / F / 7 pFUSB4_ATCC
Spectinomycin
Plate 1 / F / 8 pFUSB4_ATTC
Spectinomycin
Plate 1 / F / 9 pFUSB4_CACC
Spectinomycin
Plate 1 / F / 10 pFUSB4_CATC
Spectinomycin
Plate 1 / F / 11 pFUSB4_CCCC
Spectinomycin
Plate 1 / F / 12 pFUSB4_CCTC
Spectinomycin
Plate 1 / G / 1 pFUSB4_AACG
Spectinomycin
Plate 1 / G / 2 pFUSB4_AATG
Spectinomycin
Plate 1 / G / 3 pFUSB4_ACCG
Spectinomycin
Plate 1 / G / 4 pFUSB4_ACTG
Spectinomycin
Plate 1 / G / 5 pFUSB4_AGCG
Spectinomycin
Plate 1 / G / 6 pFUSB4_AGTG
Spectinomycin
Plate 1 / G / 7 pFUSB4_ATCG
Spectinomycin
Plate 1 / G / 8 pFUSB4_ATTG
Spectinomycin
Plate 1 / G / 9 pFUSB4_CACG
Spectinomycin
Plate 1 / G / 10 pFUSB4_CATG
Spectinomycin
Plate 1 / G / 11 pFUSB4_CCCG
Spectinomycin
Plate 1 / G / 12 pFUSB4_CCTG
Spectinomycin
Plate 1 / H / 1 pFUSB4_AACT
Spectinomycin
Plate 1 / H / 2 pFUSB4_AATT
Spectinomycin
Plate 1 / H / 3 pFUSB4_ACCT
Spectinomycin
Plate 1 / H / 4 pFUSB4_ACTT
Spectinomycin
Plate 1 / H / 5 pFUSB4_AGCT
Spectinomycin
Plate 1 / H / 6 pFUSB4_AGTT
Spectinomycin
Plate 1 / H / 7 pFUSB4_ATCT
Spectinomycin
Plate 1 / H / 8 pFUSB4_ATTT
Spectinomycin
Plate 1 / H / 9 pFUSB4_CACT
Spectinomycin
Plate 1 / H / 10 pFUSB4_CATT
Spectinomycin
Plate 1 / H / 11 pFUSB4_CCCT
Spectinomycin
Plate 1 / H / 12 pFUSB4_CCTT
Spectinomycin
Plate 2 / A / 1 pFUSB4_CGAA
Spectinomycin
Plate 2 / A / 2 pFUSB4_CGGA
Spectinomycin
Plate 2 / A / 3 pFUSB4_CTAA
Spectinomycin
Plate 2 / A / 4 pFUSB4_CTGA
Spectinomycin
Plate 2 / A / 5 pFUSB4_GAAA
Spectinomycin
Plate 2 / A / 6 pFUSB4_GAGA
Spectinomycin
Plate 2 / A / 7 pFUSB4_GCAA
Spectinomycin
Plate 2 / A / 8 pFUSB4_GCGA
Spectinomycin
Plate 2 / A / 9 pFUSB4_GGAA
Spectinomycin
Plate 2 / A / 10 pFUSB4_GGGA
Spectinomycin
Plate 2 / A / 11 pFUSB4_GTAA
Spectinomycin
Plate 2 / A / 12 pFUSB4_GTGA
Spectinomycin
Plate 2 / B / 1 pFUSB4_CGAC
Spectinomycin
Plate 2 / B / 2 pFUSB4_CGGC
Spectinomycin
Plate 2 / B / 3 pFUSB4_CTAC
Spectinomycin
Plate 2 / B / 4 pFUSB4_CTGC
Spectinomycin
Plate 2 / B / 5 pFUSB4_GAAC
Spectinomycin
Plate 2 / B / 6 pFUSB4_GAGC
Spectinomycin
Plate 2 / B / 7 pFUSB4_GCAC
Spectinomycin
Plate 2 / B / 8 pFUSB4_GCGC
Spectinomycin
Plate 2 / B / 9 pFUSB4_GGAC
Spectinomycin
Plate 2 / B / 10 pFUSB4_GGGC
Spectinomycin
Plate 2 / B / 11 pFUSB4_GTAC
Spectinomycin
Plate 2 / B / 12 pFUSB4_GTGC
Spectinomycin
Plate 2 / C / 1 pFUSB4_CGAG
Spectinomycin
Plate 2 / C / 2 pFUSB4_CGGG
Spectinomycin
Plate 2 / C / 3 pFUSB4_CTAG
Spectinomycin
Plate 2 / C / 4 pFUSB4_CTGG
Spectinomycin
Plate 2 / C / 5 pFUSB4_GAAG
Spectinomycin
Plate 2 / C / 6 pFUSB4_GAGG
Spectinomycin
Plate 2 / C / 7 pFUSB4_GCAG
Spectinomycin
Plate 2 / C / 8 pFUSB4_GCGG
Spectinomycin
Plate 2 / C / 9 pFUSB4_GGAG
Spectinomycin
Plate 2 / C / 10 pFUSB4_GGGG
Spectinomycin
Plate 2 / C / 11 pFUSB4_GTAG
Spectinomycin
Plate 2 / C / 12 pFUSB4_GTGG
Spectinomycin
Plate 2 / D / 1 pFUSB4_CGAT
Spectinomycin
Plate 2 / D / 2 pFUSB4_CGGT
Spectinomycin
Plate 2 / D / 3 pFUSB4_CTAT
Spectinomycin
Plate 2 / D / 4 pFUSB4_CTGT
Spectinomycin
Plate 2 / D / 5 pFUSB4_GAAT
Spectinomycin
Plate 2 / D / 6 pFUSB4_GAGT
Spectinomycin
Plate 2 / D / 7 pFUSB4_GCAT
Spectinomycin
Plate 2 / D / 8 pFUSB4_GCGT
Spectinomycin
Plate 2 / D / 9 pFUSB4_GGAT
Spectinomycin
Plate 2 / D / 10 pFUSB4_GGGT
Spectinomycin
Plate 2 / D / 11 pFUSB4_GTAT
Spectinomycin
Plate 2 / D / 12 pFUSB4_GTGT
Spectinomycin
Plate 2 / E / 1 pFUSB4_CGCA
Spectinomycin
Plate 2 / E / 2 pFUSB4_CGTA
Spectinomycin
Plate 2 / E / 3 pFUSB4_CTCA
Spectinomycin
Plate 2 / E / 4 pFUSB4_CTTA
Spectinomycin
Plate 2 / E / 5 pFUSB4_GACA
Spectinomycin
Plate 2 / E / 6 pFUSB4_GATA
Spectinomycin
Plate 2 / E / 7 pFUSB4_GCCA
Spectinomycin
Plate 2 / E / 8 pFUSB4_GCTA
Spectinomycin
Plate 2 / E / 9 pFUSB4_GGCA
Spectinomycin
Plate 2 / E / 10 pFUSB4_GGTA
Spectinomycin
Plate 2 / E / 11 pFUSB4_GTCA
Spectinomycin
Plate 2 / E / 12 pFUSB4_GTTA
Spectinomycin
Plate 2 / F / 1 pFUSB4_CGCC
Spectinomycin
Plate 2 / F / 2 pFUSB4_CGTC
Spectinomycin
Plate 2 / F / 3 pFUSB4_CTCC
Spectinomycin
Plate 2 / F / 4 pFUSB4_CTTC
Spectinomycin
Plate 2 / F / 5 pFUSB4_GACC
Spectinomycin
Plate 2 / F / 6 pFUSB4_GATC
Spectinomycin
Plate 2 / F / 7 pFUSB4_GCCC
Spectinomycin
Plate 2 / F / 8 pFUSB4_GCTC
Spectinomycin
Plate 2 / F / 9 pFUSB4_GGCC
Spectinomycin
Plate 2 / F / 10 pFUSB4_GGTC
Spectinomycin
Plate 2 / F / 11 pFUSB4_GTCC
Spectinomycin
Plate 2 / F / 12 pFUSB4_GTTC
Spectinomycin
Plate 2 / G / 1 pFUSB4_CGCG
Spectinomycin
Plate 2 / G / 2 pFUSB4_CGTG
Spectinomycin
Plate 2 / G / 3 pFUSB4_CTCG
Spectinomycin
Plate 2 / G / 4 pFUSB4_CTTG
Spectinomycin
Plate 2 / G / 5 pFUSB4_GACG
Spectinomycin
Plate 2 / G / 6 pFUSB4_GATG
Spectinomycin
Plate 2 / G / 7 pFUSB4_GCCG
Spectinomycin
Plate 2 / G / 8 pFUSB4_GCTG
Spectinomycin
Plate 2 / G / 9 pFUSB4_GGCG
Spectinomycin
Plate 2 / G / 10 pFUSB4_GGTG
Spectinomycin
Plate 2 / G / 11 pFUSB4_GTCG
Spectinomycin
Plate 2 / G / 12 pFUSB4_GTTG
Spectinomycin
Plate 2 / H / 1 pFUSB4_CGCT
Spectinomycin
Plate 2 / H / 2 pFUSB4_CGTT
Spectinomycin
Plate 2 / H / 3 pFUSB4_CTCT
Spectinomycin
Plate 2 / H / 4 pFUSB4_CTTT
Spectinomycin
Plate 2 / H / 5 pFUSB4_GACT
Spectinomycin
Plate 2 / H / 6 pFUSB4_GATT
Spectinomycin
Plate 2 / H / 7 pFUSB4_GCCT
Spectinomycin
Plate 2 / H / 8 pFUSB4_GCTT
Spectinomycin
Plate 2 / H / 9 pFUSB4_GGCT
Spectinomycin
Plate 2 / H / 10 pFUSB4_GGTT
Spectinomycin
Plate 2 / H / 11 pFUSB4_GTCT
Spectinomycin
Plate 2 / H / 12 pFUSB4_GTTT
Spectinomycin
Plate 3 / A / 1 pFUSB4_TAAA
Spectinomycin
Plate 3 / A / 2 pFUSB4_TAGA
Spectinomycin
Plate 3 / A / 3 pFUSB4_TCAA
Spectinomycin
Plate 3 / A / 4 pFUSB4_TCGA
Spectinomycin
Plate 3 / A / 5 pFUSB4_TGAA
Spectinomycin
Plate 3 / A / 6 pFUSB4_TGGA
Spectinomycin
Plate 3 / A / 7 pFUSB4_TTAA
Spectinomycin
Plate 3 / A / 8 pFUSB4_TTGA
Spectinomycin
Plate 3 / A / 9 pFUSB4_TTTT
Spectinomycin
Plate 3 / A / 10 pFUSB4_TTCT
Spectinomycin
Plate 3 / A / 11 pFUSB4_TGTT
Spectinomycin
Plate 3 / A / 12 pFUSB4_TGCT
Spectinomycin
Plate 3 / B / 1 pFUSB4_TAAC
Spectinomycin
Plate 3 / B / 2 pFUSB4_TAGC
Spectinomycin
Plate 3 / B / 3 pFUSB4_TCAC
Spectinomycin
Plate 3 / B / 4 pFUSB4_TCGC
Spectinomycin
Plate 3 / B / 5 pFUSB4_TGAC
Spectinomycin
Plate 3 / B / 6 pFUSB4_TGGC
Spectinomycin
Plate 3 / B / 7 pFUSB4_TTAC
Spectinomycin
Plate 3 / B / 8 pFUSB4_TTGC
Spectinomycin
Plate 3 / B / 9 pFUSB4_TACT
Spectinomycin
Plate 3 / B / 10 pFUSB4_TATT
Spectinomycin
Plate 3 / B / 11 pFUSB4_TCCT
Spectinomycin
Plate 3 / B / 12 pFUSB4_TCTT
Spectinomycin
Plate 3 / C / 1 pFUSB4_TAAG
Spectinomycin
Plate 3 / C / 2 pFUSB4_TAGG
Spectinomycin
Plate 3 / C / 3 pFUSB4_TCAG
Spectinomycin
Plate 3 / C / 4 pFUSB4_TCGG
Spectinomycin
Plate 3 / C / 5 pFUSB4_TGAG
Spectinomycin
Plate 3 / C / 6 pFUSB4_TGGG
Spectinomycin
Plate 3 / C / 7 pFUSB4_TTAG
Spectinomycin
Plate 3 / C / 8 pFUSB4_TTGG
Spectinomycin
Plate 3 / C / 9 pFUSB4_TACG
Spectinomycin
Plate 3 / C / 10 pFUSB4_TATG
Spectinomycin
Plate 3 / C / 11 pFUSB4_TCCG
Spectinomycin
Plate 3 / C / 12 pFUSB4_TCTG
Spectinomycin
Plate 3 / D / 1 pFUSB4_TAAT
Spectinomycin
Plate 3 / D / 2 pFUSB4_TAGT
Spectinomycin
Plate 3 / D / 3 pFUSB4_TCAT
Spectinomycin
Plate 3 / D / 4 pFUSB4_TCGT
Spectinomycin
Plate 3 / D / 5 pFUSB4_TGAT
Spectinomycin
Plate 3 / D / 6 pFUSB4_TGGT
Spectinomycin
Plate 3 / D / 7 pFUSB4_TTAT
Spectinomycin
Plate 3 / D / 8 pFUSB4_TTGT
Spectinomycin
Plate 3 / D / 9 pFUSB4_TGCG
Spectinomycin
Plate 3 / D / 10 pFUSB4_TGTG
Spectinomycin
Plate 3 / D / 11 pFUSB4_TTCG
Spectinomycin
Plate 3 / D / 12 pFUSB4_TTTG
Spectinomycin
Plate 3 / E / 1 pFUSB4_TACA
Spectinomycin
Plate 3 / E / 2 pFUSB4_TATA
Spectinomycin
Plate 3 / E / 3 pFUSB4_TCCA
Spectinomycin
Plate 3 / E / 4 pFUSB4_TCTA
Spectinomycin
Plate 3 / E / 5 pFUSB4_TGCA
Spectinomycin
Plate 3 / E / 6 pFUSB4_TGTA
Spectinomycin
Plate 3 / E / 7 pFUSB4_TTCA
Spectinomycin
Plate 3 / E / 8 pFUSB4_TTTA
Spectinomycin
Plate 3 / E / 9 pFUSB4_TGCC
Spectinomycin
Plate 3 / E / 10 pFUSB4_TGTC
Spectinomycin
Plate 3 / E / 11 pFUSB4_TTCC
Spectinomycin
Plate 3 / E / 12 pFUSB4_TTTC
Spectinomycin
Plate 3 / F / 1 pFUSB4_TACC
Spectinomycin
Plate 3 / F / 2 pFUSB4_TATC
Spectinomycin
Plate 3 / F / 3 pFUSB4_TCCC
Spectinomycin
Plate 3 / F / 4 pFUSB4_TCTC
Spectinomycin
Data calculated @ 2017-08-22

Kit Plate Map - #1000000038

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