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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: BSB-

Source/Vendor: ATCC
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 3210
Created by Moore, July 1995, under contract with NCBI. Constructed from Bluescribe M13- by insertion of a synthetic BstXI adapter between the PstI & XbaI sites of the polylinker region. A plasmid vector containing convenient priming sites for sequence analysis and synthesis of RNA in either direction from T3 and T7 promoters. BSB+ (away from cloning site) and BSB- (toward cloning site) differ in their orientation of the intergenic region from bacteriophage f1. Contains the intergenic region from bacteriophage f1 permitting synthesis of single-strand copies of the insert. Restriction digests of the clone give the following sizes (kb): NdeI/HindIII--2.45, 0.75; BamHI--3.2; NdeI--3.2; PvuII--2.65 + smaller; PstI--uncut. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli. Related vectors: BlueScribe M13-. (Information source: VectorDB ( ).)
Catalog Number: 67725
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map