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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: EBO-pSV2-neo

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 13360
Notes:
The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive. [1] pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA. [1] A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells; maintained episomally. [1] Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH1, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number: 37593
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified