Vector Database

The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive. [1] pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA. [1] A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells; maintained episomally. [1] Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH1, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)