Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- The vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive.  pcD cDNA libraries can be converted into EBO-pcD libraries by ligating the 7.4 kb SfiI fragment of EBO-pSV2-neo (containing oriP, EBNA-1, hph) into SfiI-linearized, phosphatased, pcD DNA.  A selectable, stably replicating shuttle vector for expression of cDNAs in eukaryotic cells; maintained episomally.  Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--13.4; SfiI--7.5, 6.1; PstI--7.4, 2.3, 1.5, 1.0; SacI--13.4; BamHI--6.8, 5.4; HindIII--13.4; BglII--6.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH1, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number