Vector Database

Restriction digests of the clone give the following sizes (kb): EcoRI--33.5, 14.5; BamHI--33.5, 14.5. (ATCC staff) For library construction, a double digest of the vector with BamHI + EcoRI and removal of the linker fragment reduces the number of background phage. Phosphatase treatment of digested vector eliminates background. (personal communication) By labelling the left cohesive end, which is 200 bp from cloning sites, reliable restriction maps of clones can be made from partial digests. (personal communication) A modified EMBL vector, with all phage coding sequences to the right of the cloning sites to facilitate restriction mapping of clones. Can be used for genomic library construction. (personal communication) Medium is 1592 SM buffer. Hosts: bacteria-free lysate, E.coli MC1061, E.coli K802. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)