Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: EMSV
| Alt Name: | pEMSVscribe |
| Plasmid Type: | Mammalian Expression |
| Cloning Method: | Unknown |
| Size: | 5300 |
| Bacterial Resistance: | Ampicillin |
| Selectable Marker: | Puromycin |
| Notes: | Davis RL, Weintraub H, Lassar AB., Cell. 1987 Dec 24;51(6):987-1000.
pEMSVscribe was
generated by first digesting Bluescribe M13(+) (Stratagene) with
EcoRI, filling in the ends with the Klenow fragment of E. coli pol I
(Boehringer Mannheim), and religating to create Bluescribe
M13(+)EcoRI-. This vector was then cut with Hindlll, and the Hindlll
fragment of EMSV-33 (Harland and Weintraub, 1985) containing the
Moloney sarcoma virus LTR, and SV40 poly(A) addition signal was inserted
by religation. This construct allows unique EcoRl insertion between
the LTR and poly(A) signal sequence, which are themselves
flanked by T7 and T3 promoters.
More information: Davis RL, Weintraub H, Lassar AB., Cell. 1987 Dec 24;51(6):987-1000. |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
| Viral/Non-Viral: | Unspecified |