Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
|Plasmid Type:||Mammalian Expression|
Davis RL, Weintraub H, Lassar AB., Cell. 1987 Dec 24;51(6):987-1000. pEMSVscribe was generated by first digesting Bluescribe M13(+) (Stratagene) with EcoRI, filling in the ends with the Klenow fragment of E. coli pol I (Boehringer Mannheim), and religating to create Bluescribe M13(+)EcoRI-. This vector was then cut with Hindlll, and the Hindlll fragment of EMSV-33 (Harland and Weintraub, 1985) containing the Moloney sarcoma virus LTR, and SV40 poly(A) addition signal was inserted by religation. This construct allows unique EcoRl insertion between the LTR and poly(A) signal sequence, which are themselves flanked by T7 and T3 promoters. More information: Davis RL, Weintraub H, Lassar AB., Cell. 1987 Dec 24;51(6):987-1000.