Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: KS+SV0CAT
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4841
- Notes
- Created by Moore, July 1995, under contract with NCBI. HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI sites. Mammalian shuttle expression vector for promoter cloning with activity analysis by CAT (chloramphenicol acetyl transferase) assay. Use KS+SV2CAT (ATCC 37609) as a control. Restriction digests of the clone give the following sizes (kb): SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4; EcoRI--3.9, 0.32; HindIII--4.4. (ATCC staff) Constructed by inserting the HindIII-ApaI fragment of pSV2cat in HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. Related vectors: pBluescript KS+, pSV2-cat. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37608
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified