Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Created by Moore, July 1995, under contract with NCBI. HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI sites. Mammalian shuttle expression vector for promoter cloning with activity analysis by CAT (chloramphenicol acetyl transferase) assay. Use KS+SV2CAT (ATCC 37609) as a control. Restriction digests of the clone give the following sizes (kb): SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4; EcoRI--3.9, 0.32; HindIII--4.4. (ATCC staff) Constructed by inserting the HindIII-ApaI fragment of pSV2cat in HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. Related vectors: pBluescript KS+, pSV2-cat. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)