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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Created by Moore, July 1995, under contract with NCBI. Mammalian shuttle expression vector containing a CAT (chloramphenicol acetyl transferase) gene expressed under an SV40 promoter. To be used as a control for promoter activity in conjuction with the promoter cloning plasmid KS+SV0CAT (ATCC 37608). Additional polylinker sites are ApaI and KpnI. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 2.1; SmaI--4.7; PstI--4.7; EcoRI--4.l, 0.72; ClaI--4.7. (ATCC staff) Constructed by inserting the AccI-ApaI fragment of pSV2cat into the EcoRV site of Bluescript KS+. Ends were blunt. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli, vertebrate cells. Related vectors: pBluescript KS+, pSV2-cat. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number