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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: KS+SV2CAT

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
5373
Notes
Created by Moore, July 1995, under contract with NCBI. Mammalian shuttle expression vector containing a CAT (chloramphenicol acetyl transferase) gene expressed under an SV40 promoter. To be used as a control for promoter activity in conjuction with the promoter cloning plasmid KS+SV0CAT (ATCC 37608). Additional polylinker sites are ApaI and KpnI. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 2.1; SmaI--4.7; PstI--4.7; EcoRI--4.l, 0.72; ClaI--4.7. (ATCC staff) Constructed by inserting the AccI-ApaI fragment of pSV2cat into the EcoRV site of Bluescript KS+. Ends were blunt. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli, vertebrate cells. Related vectors: pBluescript KS+, pSV2-cat. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37609
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified

Sequence