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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: lambda 2001

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
41900
Notes
Rehydrate in 1 ml 10 mM magnesium sulphate. Recombinants are Spi- cI- Gam- Int- Red-. A lambda replacement vector carrying a polylinker sequence with positive selection for large (10-23 kb) inserts.) Genotype of vector is lambdasbhI lambda1(0)b189 SIGMAint (linker) srIlambda3(0) ninL44 SIGMAshndIII lambda4 (bio) (linker) delta(sbhIlambda3-sbhIlambda4) KH54 srIlambda4(0) chiC nin5 srIlambda5(0) shndIIIlambda6(0). The arms are 20.1 kb and 9.4 kb. (ATCC staff) Medium is 1592 SM buffer. Hosts: bacteria-free lysate, E.coli, E.coli LE392 [for propagation], E.coli NM539 [for recombinant]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37331
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified