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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: lambda 2001
- Plasmid Type
- Cloning Method
- Rehydrate in 1 ml 10 mM magnesium sulphate. Recombinants are Spi- cI- Gam- Int- Red-. A lambda replacement vector carrying a polylinker sequence with positive selection for large (10-23 kb) inserts.) Genotype of vector is lambdasbhI lambda1(0)b189 SIGMAint (linker) srIlambda3(0) ninL44 SIGMAshndIII lambda4 (bio) (linker) delta(sbhIlambda3-sbhIlambda4) KH54 srIlambda4(0) chiC nin5 srIlambda5(0) shndIIIlambda6(0). The arms are 20.1 kb and 9.4 kb. (ATCC staff) Medium is 1592 SM buffer. Hosts: bacteria-free lysate, E.coli, E.coli LE392 [for propagation], E.coli NM539 [for recombinant]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number