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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: lambda NMT

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
38000
Notes
Vector for efficient transduction of mammalian cells with a cDNA library constructed with the pcD (Okayama and Berg) expression vector. The order of the major features in this bacteriophage is: lambda762/deltab - HindIII - 0.5 kb SV40 fragment, map units 0.86-0.95 - reddelta - SalI - SV40 ori - SV40 early promoter - SV40 late 19S and 16S splice junctions - neo (Tn5) - SV40 late poly(A) - pMB1 ori - ampR - lambda762/KH54/lambda ori/nin5/QSR. Cloning capacity:12 kb, or 9 kb of cDNA insert and 3 kb of pcD vector. Medium is 1592 SM buffer. Hosts: bacteria-free lysate, vertebrate cells, E.coli, E.coli JC8679 [ATCC 47001 for library amplification], E.coli C600 [ATCC 23724 for vector amplification]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37367
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified