Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: lambda ZD32
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 43700
- Notes
- Restriction digests of the clone give the following sizes (kb): EcoRI--18.5, 26.0. (ATCC staff) Same as lambdaZd31 but the tri-initiator is in the opposite direction. A cell line suitable for transfection by released plasmid is Wgd5, ATCC CRL 1817. The plasmid insert can be released by homologous recombination between the 2 LTRs after growth at low temperature in E.coli RRI(pRK248), ATCC 47036. Allows expression of cloned cDNA in mammalian cells; can be used for library transduction. Medium is 1592 SM buffer. Hosts: bacteria-free lysate, vertebrate cells, E.coli, E.coli RR1 pRK248 [ATCC 47036], E.coli Wgd5 [CRL 1817]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37564
- GenBank
- M22642, M19164, M19165, M19166, M19167, M19168
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified