Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: lambdaSK17
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 51000
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--25.0, 13.5 (doublet); EcoRI--25.0, 13.5 (doublet); SalI--21.0, 8.8, 8.2, 5.0, 4.4, 3.6. (ATCC staff) Expression vector permitting positive selection (Spi) of recombinants and control of expression using ochre (supB, supC and/or supG, supV) and amber (supE, supF) suppressors. Useful for conversion to phagemid. Permits expression in three reading frames, construction using partial filling-in, and oriented cloning. The sizes of the major components are (kb): arms--20.3, 12.7; stuffer fragment--13.7; phagemid segments--3.9 (longer arm), 0.7. The polylinker sites given are those associated with the longer arm. The sites associated with the other arm are: SalI BamHI EcoRI XhoI NotI NaeI SfiI AvrII NheI NcoI XbaI. The restriction sites to be used for conversion to phagemid are: SalI, ClaI. Cloning capacity: 0.2 - 15.4 kb. The order of the major features in this plasmid is: arm - ClaI - BspMII - SalI - pMB1 ori - ampR - M13 ori - SacI/MCS/ SalI --> - stuffer - SalI/MCS/XbaI/lacZ' - ClaI - SalI - arm. [2] Deposited by: Zabarovsky E.R., Winberg G.R., Klein G.R. Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 86969
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified