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Vector Database


Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: M13mp8

Source/Vendor: Amersham
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 7229
Notes:
M13 is not lytic; the phage extrude through the cell wall. The replicative RF I form in the cell is double-stranded and circular, and acts like a plasmid. The phage form outside the cell is linear and single-stranded. In vitro DNA synthesis on a cis-DDP-treated template generates a population of DNA fragments, indicating that duplex synthesis has terminated. Cis-DDP forms an adduct with DNA, at positions where multiple 'g' nucleotides occur, that inhibits synthesis in vitro. Trans DDP has the same effect, only on 'gng' sequences, where n can be any intervening nucleotide. NCBI gi: 215214 & 215215 Hosts: E.coli JM101, E.coli JM103, E.coli JM105, E.coli JM107, E.coli JM109, E.coli DH5alphaF'. Related vectors: M13mp7, M13mp9. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
GenBank: M77826, M11403
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map

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