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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- M13 is not lytic; the phage extrude through the cell wall. The replicative RF I form in the cell is double-stranded and circular, and acts like a plasmid. The phage form outside the cell is linear and single-stranded. In vitro DNA synthesis on a cis-DDP-treated template generates a population of DNA fragments, indicating that duplex synthesis has terminated. Cis-DDP forms an adduct with DNA, at positions where multiple 'g' nucleotides occur, that inhibits synthesis in vitro. Trans DDP has the same effect, only on 'gng' sequences, where n can be any intervening nucleotide. NCBI gi: 215214 & 215215 Hosts: E.coli JM101, E.coli JM103, E.coli JM105, E.coli JM107, E.coli JM109, E.coli DH5alphaF'. Related vectors: M13mp7, M13mp9. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- M77826, M11403