Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
not original sequence. fusion regions. Shuttle vector conferring bleomycin and phleomycin resistance. The order of the major features in this plasmid is:pUC8 - EcoRI - BamHI - am promoter - ble - XhoI - am 3' region - HindIII - pUC8. Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa. Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7,1.5; BamHI/XhoI--3.4, 0.8; BglI--3.0, 1.2. (ATCC staff) A (GT)n repeat. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Aspergillus nidulans, Neurospora crassa, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)