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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- clone pHP3 = rabbit minor cytochrome P-450 pAHF3 contained the entire coding sequence of pHP3 (RABP45PH3), but nucleotide sequences for the N-terminal region of pHP3 protein (from the 2nd to the 23rd amino acid) were deleted in pAH-delta-N3. On introduction into yeast AH22 cells, the absorption spectrum of cytochrome P-450 was detected in the microsomal fraction from the transformed cells csrrying pAHF3. On the other hand, cytochrome P-450 could not be detected spectrophotometrically in any subcellular fractions from the yeast cells carrying pAH-delta-N3, although the transcript of pHP3 insert was detected in RNA blot analysis. NCBI gi: 220960 Hosts: yeast AH22. Related vectors: pAAH5, pHP3. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- D10362, D00192