Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pAM34
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6000
- Notes
- Permits positive selection because deletion of the aadA fragment is not viable. pAM35 (ATCC 77186) differs from pAM34 (ATCC 77185) by deletion of the DraII fragment in pAM34 carrying lacIq. The order of the major features in this plasmid is: lacZpo - ori - rom - transcription terminator- BamHI - MCS - XmaIII - aadA (spcR) - MCS - BamHI - transcription terminator - bla - lacIq. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; BamHI--4.3,1.8; HindIII--6.0; PstI--6.0; KpnI--3.4, 2.7. (ATCC staff) Medium is -1 1227 + IPTG (1 mM). Hosts: E.coli JS219, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77185
- GenBank
- M69063
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified