Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pAP12BD
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 15000
- Notes
- The promoter and terminator sequences were derived from the phosphoglycerate kinase gene. Saccharomyces cerevisiae containing a single copy of the neomycin phosphotransferase gene are sensitive to G418; resistance to 400 ug/ml is expressed if there are several copies per cell. The G418-resistance segment includes a 0.7 kb region of the PGK promoter and a 1.5 kb BglII/BamHI fragment from Tn5. The BamHI site is between the G418R and the PGK terminator. Restriction digests of the clone give the following sizes (kb): BamHI--15.0; BglII--15.0; XhoI--9.4, 6.0; EcoRI--13.0, 2.1; KpnI--13.0, 2.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JA221, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 53231
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified