Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pARO190


Plasmid Type
Cloning Method
Mobilizable expression vector encoding the lacZalpha (lacZ') peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing. Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains. Replicates to high copy number in E.coli and Enterobacter aerogenes. Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. This region can be transferred to another version of pUC vectors by using a MCS restriction site and the unique AlwNI site. The order of the major features in this plasmid is:lacZ'/MCS - pMB1 ori - AlwNI site - bla - oriT/bom. pARO180 (ATCC 77123) and pARO190 (ATCC 77124) differ in the orientation of the MCS in lacZ'. Restriction digests of the clone give the following sizes (kb): BglI--1.55, 0.9, 0.8 (doublet), 0.75, 0.7; HindIII/PvuI--3.9, 1.6; EcoRI/PvuI--3.9, 1.6; EcoRI--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, Enterobacter aerogenes, E.coli, E.coli S17-1 [ATCC 47055]. (Information source: VectorDB ( ).)
Catalog Number