Vector Database

Mobilizable expression vector encoding the lacZalpha (lacZ') peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing. Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains. Replicates to high copy number in E.coli and Enterobacter aerogenes. Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. This region can be transferred to another version of pUC vectors by using a MCS restriction site and the unique AlwNI site. The order of the major features in this plasmid is:lacZ'/MCS - pMB1 ori - AlwNI site - bla - oriT/bom. pARO180 (ATCC 77123) and pARO190 (ATCC 77124) differ in the orientation of the MCS in lacZ'. Restriction digests of the clone give the following sizes (kb): BglI--1.55, 0.9, 0.8 (doublet), 0.75, 0.7; HindIII/PvuI--3.9, 1.6; EcoRI/PvuI--3.9, 1.6; EcoRI--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DH5alpha, Enterobacter aerogenes, E.coli, E.coli S17-1 [ATCC 47055]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)