Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pAS1
| Source/Vendor: | ATCC |
| Plasmid Type: | Unspecified |
| Cloning Method: | Unknown |
| Size: | 7100 |
| Notes: | Restriction digests of the clone give the following sizes (kb): BamHI--7.2; SalI--7.2; SmaI--7.2. (ATCC staff) Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. The order of the major features in this plasmid is: ADC1 (ADH) promoter -> - GAL4 DNA binding domain - HA - EcoRI - NdeI/MCS/SalI - ADC1 terminator - pMB1 ori - ampR - 2 micron ori - TRP1 -> - f1 ori. [1] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Elledge S.J. Hosts: E.coli, Saccharomyces cerevisiae. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
| Catalog Number: | 87007 |
| Stable: | Unspecified |
| Constitutive: | Unspecified |
| Viral/Non-Viral: | Unspecified |