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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pAS2

Source/Vendor: ATCC
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 8500
Restriction digests of the clone give the following sizes (kb): BamHI--8.6; SalI--8.6; SmaI--8.6. (ATCC staff) Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. [1] The order of the major features in this plasmid is: ADC1 (ADH) promoter -> - GAL4 DNA binding domain - HA - NdeI/MCS/SalI - ADC1 terminator - pMB1 ori - ampR - 2 micron ori - TRP1 -> - f1 ori - <- CYH2. [2] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Elledge S.J. Hosts: E.coli, yeast, Saccharomyces cerevisiae. (Information source: VectorDB ( ).)
Catalog Number: 87008
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified