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Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pASZ11


Plasmid Type
Cloning Method
The ADE2 segment contains HindIII sites approximately 0.9 and 2.1 kb from the 5' BglII site. and the naturally-occurring BglII site at the 3' end (nt 2512) was used to create a BglII fragment containing the complete coding sequence. The naturally-occurring BglII site within ADE2 was destroyed by replacing the A at nt 1243 of the reported sequence with a G. A BglII linker was added to the 5' end. Constructed by replacing the TRP1-containing BglII fragment of pFL39 with a 2.2 kb fragment (Ars- Ade2+). In E.coli, permits visual detection of recombinants. Encodes the lacZalpha (lacZ') peptide. A YC-type shuttle expression vector containing priming sites useful for sequencing and readily purifiable elements for construction experiments. pFL39 was derived from pUC19 by inserting a ClaI fragment containing ARS-CEN at nt 747 and BglII-linkered fragment containing TRP1 at nt 629. The order of the major features in this plasmid is:lacZ'/MCS - pMB1 ori - ADE2 - ARS-CEN. Restriction digests of the clone give the following sizes (kb): BglII--3.5, 2.3; EcoRI--5.8; HindIII--3.5, 1.25, 1.15; BamHI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( ).)
Catalog Number
M59824, M32013