Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
The ADE2 segment contains HindIII sites approximately 0.9 and 2.1 kb from the 5' BglII site. and the naturally-occurring BglII site at the 3' end (nt 2512) was used to create a BglII fragment containing the complete coding sequence. The naturally-occurring BglII site within ADE2 was destroyed by replacing the A at nt 1243 of the reported sequence with a G. A BglII linker was added to the 5' end. Constructed by replacing the TRP1-containing BglII fragment of pFL39 with a 2.2 kb fragment (Ars- Ade2+). In E.coli, permits visual detection of recombinants. Encodes the lacZalpha (lacZ') peptide. A YC-type shuttle expression vector containing priming sites useful for sequencing and readily purifiable elements for construction experiments. pFL39 was derived from pUC19 by inserting a ClaI fragment containing ARS-CEN at nt 747 and BglII-linkered fragment containing TRP1 at nt 629. The order of the major features in this plasmid is:lacZ'/MCS - pMB1 ori - ADE2 - ARS-CEN. Restriction digests of the clone give the following sizes (kb): BglII--3.5, 2.3; EcoRI--5.8; HindIII--3.5, 1.25, 1.15; BamHI--5.8. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)