Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Shares pBR322 sequences from the PvuII site to the ScaI site with pcD. Plasmid linearized with PstI can be used to introduce cDNA libraries constructed in pcD or pcD2 vectors into Schizosaccharomyces pombe, resulting in the formation, by homologous recombination, of concatamers maintained as episomal DNA. Contains the ars sequence from Schizosaccharomyces pombe and the URA3 gene from Saccharomyces cerevisiae. Insertions at the BamHI or SalI sites inactivate tetracycline resistance. Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII--2.6 (doublet); PstI--5.2. (ATCC staff) Medium is 1273 LB plus tetracycline. Hosts: E.coli HB101, Schizosaccharomyces pombe, E.coli, Schizosaccharomyces pombe [ATCC 38436]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number