Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- ATPG (para-aminophenyl-1-thio-beta-D-galactopyranoside) is like ITPG. Cleave away beta-galactosidase with collagenase or endoproteinase Xa. + makes non-coding strand. - makes coding strand. NruI site after Xa site allows purifying cloned protein with no additional N-terminal amino acids. Hosts: E.coli. Related vectors: pBR322, f1, pAX4a-, pAX4b+, pAX4b-, pAX4c+, pAX4c-, pAX4a+, pAX5-. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)