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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pBC12MI

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
4100
Notes
This was constructed from pXF3 (pBR322 minus the poison sequence), a SV40 ori, and a rat preproinsulin genomic sequence under the control of RSV LTR (pBC81delta4 cut at BglII and BalI and inserted at the BamHI and ClaI sites). Restriction digests of the clone give the following sizes (kb): EcoRI--2.85, 0.76; HindIII--4.1; BamHI--4.1. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli MC1061, vertebrate cells, E.coli. Related vectors: pXF3 from pBR322. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
67109
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified