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Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pBCL8.1

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
8100
Notes
Chromosome acentric fragmentation vector containing LYS2 for use with artificial chromosomes containing Alu repeats. pBCL8.1 should be linearized with SalI before transformation into human YAC-containing yeast strains. Lys+ Trp+ Ura- colonies should be selected. Recombinant deletion YACs can be recovered by digestion with BglII, EcoRI, HindIII, PstI, SacI or XbaI, recircularization, and transformation into E. coli. The order of the major features in the plasmid is: LYS2 - HindIII - telomere - SalI - Alu repeats - pMB1 ori - ampR - SacI - XbaI - PstI - EcoRI. [1] Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: C.T.Denny Hosts: Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
77418
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified