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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Permits positive selection for inserts, based on the dominance of the wild-type rpsL (ribosomal protein S12) gene in the plasmid. The SmaI and one HpaI site are in this gene. (ATCC staff) Derived from pNO1523 by inserting a BglII linker at the EcoRI site and a HpaI linker at the PvuII site. (personal communication) The order of the major features in this plasmid is:EcoRI - BglII - EcoRI - ClaI - HindIII - BamHI - rpsL - ori - bla. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; BglII--5.2; HindIII--5.2; PstI--3.6, 1.6. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul). Hosts: E.coli RR1, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number