Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pBJ1-Neo
Information
- Alt Name
- pBJ1
- Plasmid Type
- Mammalian Expression
- Promoter
- SR alpha
- Cloning Method
- Unknown
- Size
- 6600
- 5' Sequencing 1 Primer
- SV40pro-F
- Bacterial Resistance
- Ampicillin
- Selectable Marker
- Neomycin
- Notes
- From Mark Davis lab: Lin et. al. Science 1990, 249 (4969) 677-679. pBJ1-Neo is a derivative of pcDL-SR alpha296 [Y. Takebe et al., MCB 8, 466 (1988)] in which an Xho I and Xho I/Bam HI fragment located between the SR alpha (human T cell lymphotropic virus type 1) promoter and the SV40 polyadenylation site has been replaced for convenient cDNA cloning by a polylinker that contains the following restriction sites: 5'-Xho I-Xba I-Sfi I-Not I- Eco RI-Eco RV- Hind III-Cla I-3'. In addition, a neomycin gene (which confers resistance to the antibiotic G418) was inserted in between the ampicillin resistance gene and the SR alpha promoter at the Pvu I site. More information: From Mark Davis lab: Lin et. al. Science 1990, 249 (4969) 677-679.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified