Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- YC-type shuttle vector designed to clone yeast genes that are difficult to clone by rescuing inserts from the vector lambdaMG3 by homologous recombination. S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype. Constructed by insertion of a 0.858 kb BamHI-XbaI fragment and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of pRS316 (ATCC 77145). The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3.  Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: M.N.Johnston Hosts: Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number