Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pBP81
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 6200
- Notes
- Constructed by replacing the telomere-adjacent sequence from pBP63A with a 0.25 kb SalI/SphI fragment containing the YTCA-1x telomere and CEN6 sequences. [1] Linearize with SalI (site adjacent to the telomere sequence) before transformation. [1] Contains 2 head to tail Alu sequences with the 5' end closer to the SalI site. [1] Centric chromosome fragmentation vector targeting Alu sequences. [1] Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.35, 0.25; PstI--4.4, 1.4, 0.4; SstI--5.4, 0.8; SalI/SphI--6.2. (ATCC staff) The order of the major features in this plasmid is: bla - HIS3 - 3'Alu5' - 3'Alu5' - SalI - TEL - CEN6. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH252, Saccharomyces cerevisiae CGY2516 [ATCC 74013]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77086
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified