Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- YI-type shuttle integrative vector targeting human LINE sequences in YAC libraries derived from somatic cell hybrids.  Constructed by replacing a 2.2 kb XbaI fragment of the LINE element in pL1.1a with a blunt-ended 1.2 kb fragment containing HIS3.  Linearizing with NotI and ClaI removes plasmid sequences from the LINE ends for targeting integration.  The NotI site is at the 5' end of the LINE sequence.  The replaced element, nt 3392 to 5607 of the LINE consensus sequence, contained conserved sequences which might have permitted targeting to murine-derived YACs.  Restriction digests of the clone give the following sizes (kb): ClaI--9.3; HindIII--4.9, 2.55, 1.85; XbaI--uncut; NotI--9.3. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, S.cerevisiae YPH252 [ATCC 76621], S.cerevisiae CYG2516 [ATCC 74013]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number