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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- YI-type shuttle integrative vector targeting human Alu sequences in YAC libraries derived from somatic cell hybrids.  Constructed by inserting a blunt-ended 1.2 kb fragment containing S. cerevisiae HIS3 into the blunted PflMI within the Alu sequence of BLUR8.  Linearizing with BamHI removes the plasmid sequences from the Alu ends for targeting integration.  Transformation into pools from a YAC library derived from a human-mouse somatic cell hybrid, and selection for His+, resulted in an approximate 50-fold enrichment for Alu-containing YACs.  Larger YACs were preferentially selected. Some YACs demonstrated loss of Alu-containing restriction fragments suggesting internal deletions resulting when the vector targeted two Alu sequences in the YAC.  Restriction digests of the clone give the following sizes (kb): BamHI--3.0, 1.5; EcoRI--4.5; PstI--4.2 +smaller. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH252, Saccharomyces cerevisiae CGY2516 [ATCC 74013]. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number