Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: pBR325


Plasmid Type
Cloning Method
These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Revised 16-DEC-1986 by F. Pfeiffer: There is a sequence conflict in that pMAC58 contains a TTG sequence where its parent pBR325 at pos 5264-5265 only contains a TG. It is unclear, if this is a mutation in pMAC58, or if the sequence of pBR325 is incorrect at that position. The plasmid pBR325 is a cloning vector constructed in vitro by the addition of the chloramphenicol resistance gene of an IS1-flanked transposon to pBR322. Sequence analysis suggests that this transposon is related to transposons Tn9 and Tn981 but is longer. No portion of insertion sequence IS1 was found in the genome. This sequence was reconstructed from the genome of pBR322, transposons Tn9 and Tn981, and pBR325 genome fragments. The actual pBR325 and pBR328 genomes may differ from this sequence. In pMAC58, a pBR325-derived vector, a TTG sequence was found where pBR325 has only a TG (pos 5264-65). It is not known if this discrepancy is a mutation or a sequence error in pBR325. Size is 5927 bp. for Amersham. Size is 5996 bp. for BRL. Hosts: E.coli HB101. Related vectors: pBR322, pBR327, pBR328. (Information source: VectorDB ( ).)