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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pBS10

Source/Vendor: NCRR YRC
Analyze: Sequence
Plasmid Type: Unspecified
Cloning Method: Unknown
Size: 4875
Plasmid pBS10 is based upon pBS4, where CFP has been mutated to the Cerulean variant. Those mutations are described in An Improved Cyan Fluorescent Protein Variant Useful for FRET by Rizzo, Springer, Granada, and Piston in Nat Biotechnol 2004;22:445-9. In addition to the mutations described we also needed to reverted the N164H and L231H found in our CFP but not the ECFP they used. Some common restriction enzyme sites are shown. Unique sites are in red. The forward and reverse primers are the same as in pDH3.
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Generated Plasmid Map