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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Expression vector based on a bacteriophage T7 RNA polymerase/promoter system and allowing visual detection of inserts using lacZalpha complementation. The T7 promoter sequence has a T->A mutation at the 5' end that does not appear to affect expression. The order and orientations of the major features of the plasmid are: <- lacZ' - HindIII - T7 promoter -> - NdeI/MCS/EcoRI - <-pMB1 ori - <- ampR.  Growth media: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: I.N.Maruyama Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number