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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pcDL-SRa296

Alt Name: pcDL-SRalpha296
Plasmid Type: Mammalian Expression
Promotor: SRalpha
Cloning Method: Unknown
Size: 3700
See Takebe et al, 1988, MCB 8: 466-472. This vector is composed of (i) the plasmid linker (HindIII-PstI) segment (802 bp) which provides the SRa promoter and SV40 late-gene splicing junction; (ii) the vector-primer (KpnI-HindIII) segment (2,606 bp) which comprises the KpnI-oligo(dT) priming site, SV40 late-region polyadenylation signal [poly(A)], and pUC plasmid-derived vector sequence (the pBR322 replication origin and beta-lactamase [Ap^R] gene lacking a PstI site [deltaPstI] are shown; the NdeI site in the vector sequence of this particular construct is converted to a SalI site for the convenience of excision of an entire cDNA transcriptional unit by SalI cleavage); and (iii) the intervening DNA piece (PstI-KpnI) (336 bp) between the plasmid linker and vector-primer segments, consisting of the SV40 DNA fragment flanked by the EcoRI (SV40 map position 0.754) and KpnI (map position 0.715) sites from plasmid pcDV1 and the short DNA (PstI-EcoRI) fragment from plasmid pLl. More information: See Takebe et al, 1988, MCB 8: 466-472.
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Unspecified

Published Plasmid Map