Vector Database

See Takebe et al, 1988, MCB 8: 466-472. This vector is composed of (i) the plasmid linker (HindIII-PstI) segment (802 bp) which provides the SRa promoter and SV40 late-gene splicing junction; (ii) the vector-primer (KpnI-HindIII) segment (2,606 bp) which comprises the KpnI-oligo(dT) priming site, SV40 late-region polyadenylation signal [poly(A)], and pUC plasmid-derived vector sequence (the pBR322 replication origin and beta-lactamase [Ap^R] gene lacking a PstI site [deltaPstI] are shown; the NdeI site in the vector sequence of this particular construct is converted to a SalI site for the convenience of excision of an entire cDNA transcriptional unit by SalI cleavage); and (iii) the intervening DNA piece (PstI-KpnI) (336 bp) between the plasmid linker and vector-primer segments, consisting of the SV40 DNA fragment flanked by the EcoRI (SV40 map position 0.754) and KpnI (map position 0.715) sites from plasmid pcDV1 and the short DNA (PstI-EcoRI) fragment from plasmid pLl. More information: See Takebe et al, 1988, MCB 8: 466-472.