Vector Database
Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
Plasmid: pcDL-SRa296
Alt Name: | pcDL-SRalpha296 |
Plasmid Type: | Mammalian Expression |
Promotor: | SRalpha |
Cloning Method: | Unknown |
Size: | 3700 |
Notes: | See Takebe et al, 1988, MCB 8: 466-472.
This vector is composed of (i) the plasmid linker (HindIII-PstI) segment (802 bp) which provides the SRa promoter and SV40 late-gene splicing junction; (ii) the vector-primer (KpnI-HindIII) segment (2,606
bp) which comprises the KpnI-oligo(dT) priming site, SV40 late-region polyadenylation signal [poly(A)], and pUC plasmid-derived vector
sequence (the pBR322 replication origin and beta-lactamase [Ap^R] gene lacking a PstI site [deltaPstI] are shown; the NdeI site in the vector sequence
of this particular construct is converted to a SalI site for the convenience of excision of an entire cDNA transcriptional unit by SalI cleavage);
and (iii) the intervening DNA piece (PstI-KpnI) (336 bp) between the plasmid linker and vector-primer segments, consisting of the SV40 DNA
fragment flanked by the EcoRI (SV40 map position 0.754) and KpnI (map position 0.715) sites from plasmid pcDV1 and the short DNA
(PstI-EcoRI) fragment from plasmid pLl.
More information: See Takebe et al, 1988, MCB 8: 466-472. |
Stable: | Unspecified |
Constitutive: | Unspecified |
Viral/Non-Viral: | Unspecified |
Published Plasmid Map
