Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pCDM8
Information
- Source/Vendor
- BCCM/LMBP
- Plasmid Type
- Mammalian Expression
- Promoter
- CMV
- Cloning Method
- Unknown
- Size
- 4356
- 5' Sequencing 1 Primer
- T7
- Notes
- Start of the nucleotide sequence between the Eco-pMB1 ori and the SV40 ori. pCDM8 is a shuttle vector designed for high-level transient expression of cloned cDNA in mammalian cells. The protein encoded by the cDNA can be expressed under control of the human cytomegalovirus immediate early promoter (CMV-IE) with enhancer and the SV40 polyadenylation site. The T7 promoter is used for in vitro transcription of the cDNA insert. The small HIV (human immunodeficiency virus) fragment (29 nucleotides) between the CMV and T7 promoter sequences has an enhancer function. The presence of the polyoma and SV40 origins of replication enables the vector to replicate episomally in mammalian cells expressing large T-antigen (e.g. WOB, COS cells). The supF tRNA suppressor gene is used for selection in E. coli (MC1061[p3]). The M13 origin is useful for synthesis of single-stranded DNA upon infection with the M13 helper phage. The SspI fragment (153 bp) of the 3' UTR region of the SV40 small t-antigen is missing, but without effect on the function of the vector.
- Stable
- Unspecified
- Constitutive
- Constitutive
- Viral/Non-Viral
- Nonviral