Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- Constructed by inserting a 1349 bp fragment from pMY17-3 containing the cI857 gene and tandem lambda PR and PL promoters into pUC9. The promoters are immediately upstream of the cloning sites. The sequence surrounding the cloning sites has been used to design primers for direct sequencing, including the M13 universal primer. Because cI857 is expressed by the plasmid itself, from its natural promoter PM, the vector may be used in virtually any E.coli strain. Expression vector containing primer sites useful for sequencing and encoding cI857. (ATCC staff) Restriction digests of the clone give the following sizes (kb): BamHI--4.0; BamHI/EcoRI--4.0; BglI--2.9, 1.1. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 208976 Hosts: E.coli AN1459, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number