Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- There is no XbaI site even though restriction site cluster indicates one.  This is a plasmid vector that maintains 20 copies/cell at 30C but replicates in an uncontrolled manner at 37C until cell death occurs (doubling copy number every 15 min.).  Restriction digests of the clone give the following sizes (kb): EcoRI--4.3. (ATCC staff) The vector was contructed from the following sequences (EcoRI site as nucleotide 1):0-35, multiple cloning site; 37-235, terminator; 416-1259, copA, copB; 1260-2879, repA, ori; 2880-4008, bla; 4089-4465, stability sequence.  The sequence from nucleotides 4089 to 4465 were derived from pSC101 (ATCC 3702) and can be removed from this construct by an EcoRI/SstI double digest.  Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli C600. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number